RCB Logo
RCB Logo




Building

हिन्दी / English

Dr. Sam J Mathew

Associate Professor
E-mail: sjmathew at rcb dot res dot in

  • PhD 2006, University of Cologne, Germany
  • Postdoc at University of Utah, USA
  • Associate Professor at RCB since 2019

Genetics of Vertebrate Skeletal Muscle Development, Differentiation, Stem Cell-mediated Regeneration and Disease

The vertebrate skeletal muscle is crucial to locomotion, posture, and metabolism among other functions. The adult skeletal muscle is made up of muscle fibers (myofibers), connective tissue (connective tissue and connective tissue fibroblasts) and the muscle stem cells (satellite cells). The myofibers are elongated, multinucleate cells that are the contractile units of the skeletal muscle, formed during development by fusion of myoblasts. The connective tissue connects the muscles to tendon and bone, forms an important component of the niche within which the myofibers and muscle stem cells reside and transmits the force of muscle contraction. The satellite cells are quiescent stem cells which are activated during muscle injury or disease and are crucial to muscle regeneration. Previously, our work on the mammalian skeletal muscle connective tissue fibroblasts identified the Wnt/beta-Catenin pathway transcription factor Tcf4 as the first marker for these fibroblasts and using mouse genetic experiments and in vitro co-cultures that Tcf4 and the muscle connective tissue fibroblasts are crucial for proper muscle maturation, differentiation and function.

The skeletal muscle fibers have distinct metabolic and contractile properties based on their function and location. Myofibers can be 'slow' (slow contracting, utilizing oxidative metabolism and slow to fatigue) or 'fast' (fast contracting, utilizing glycolytic metabolism and quick to fatigue). Muscles that are postural or are used for longer periods of time are generally rich in slow fibers whereas muscles that are used for short periods of time but require fast contraction are rich in fast fibers. Muscle fiber type is quite plastic and based on usage, fiber type in adults can convert from one to another. Skeletal muscle myosin heavy chains (MyHC) are the proteins that confer fiber type specific contractile properties to myofibers. There are mainly 6 MyHCs: MyHCI (slow), MyHCIIa, MyHCIIx/d, MyHCIIb (fast), MyHCembryonic and MyHCneonatal. MyHCIIa, MyHCIIx/d and MyHCIIb are expressed from neonatal stages and in the adult, MyHCI is expressed during development, neonatal stages and in the adult while MyHCembryonic and MyHCneonatal are normally expressed only during development and transiently during muscle injury or disease in the adult. I am currently trying to understand how skeletal muscle differentiation happens during embryonic development as well as during adult muscle regeneration after injury or disease using animal models and in vitro techniques.

  • Wellcome-DBT Intermediate Fellow
  • Jagriti Singh
    Junior Research Fellow (Project)
    jagriti.singh@rcb.res.in
  • Aatifa Zehra
    Junior Research Fellow
    aatifa.zehra@rcb.res.in
  • Jaydeep Sharma
    Junior Research Fellow
    jaydeep.sharma@rcb.res.in
  • Subhashni Sahu
    Senior Research Fellow
    subhashni.sahu@rcb.res.in
  • Anushree Bharadwaj
    Senior Research Fellow
    anushree@rcb.res.in
  • Shreyasi Das
    Senior Research Fellow
    shreyasi@rcb.res.in
  • Akashi Parashar
    Senior Research Fellow
    akashi.parashar@rcb.res.in
  • Pankaj Kumar
    Senior Research Fellow
    pankaj.kumar@rcb.res.in
  • Masum Saini
    India Alliance Early Career Fellow
    masum@rcb.res.in

Alumni

  • Megha Agarwal (PhD Student and Senior Research Fellow 2014-2021) – Currently Postdoctoral Fellow with Dr. Casey Gifford at the Stanford University School of Medicine, Stanford, USA.
  • Bhargab Kalita (SERB National Postdoctoral Fellow and Research Associate 2018-2021) – Currently Postdoctoral Fellow with Prof. Brian Dynlacht at the New York University School of Medicine, New York, USA
  • Shashank Khare (Senior Research Fellow 2019) – Currently PhD candidate at Institut Européen de Chimie et Biologie (IECB), Bordeaux, France.
  • Nimisha Mishra (Senior Research Fellow 2019) – Currently PhD candidate at the International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India.
  • Aakanksha Verma (Junior Research Fellow 2016-2018) – Currently PhD candidate at Weizmann Institute of Science, Rehovot, Israel.
  • Shlok Jindal (Junior Research Fellow 2016-2017) – Currently PhD candidate at the Indian Institute of Technology (IIT), Roorki, India.
  • Amit Kumar (Postdoctoral Fellow 2014-2016) – Currently Postdoctoral Fellow with Dr. Dinesh Rao at the David Geffen School of Medicine, University of California Los Angeles, California, USA.
  • Ankur Kumar (Project Assistant 2015-2016) – Currently PhD candidate at the Indian Institute of Technology (IIT), Mandi, India.
  • Shivangi Srivastava (Project Assistant 2014-2015) – Currently PhD candidate at Department of Biology and Biochemistry, University of Houston, Texas, USA.
  1. Agarwal M, Bharadwaj A, Mathew S.L. (2022) TLE4 regulates muscle stem cell quiescence and skeletal muscle differentiation Journal of Cell Science, 135:4
  2. Pradhan AK, G. Kandasamy, U. Chatterjee, A. Bharadwaj, S.J. Mathew, R.J. Dohmen, and Palanimurugan R. (2021) Ribosome-associated quality control mediates degradation of the premature translation termination product Orf1p of ODC antizyme mRNA. FEBS Letters 595: 2015-2033. doi: 10.1002/1873-3468.14147
  3. Agarwal M, A. Sharma, P. Kumar, A. Kumar, and S.J. Mathew. 2020. The people behind the papers. Development 147(7). pii: dev190025. doi: 10.1242/dev.190025.
  4. Agarwal M, A. Sharma, P. Kumar, A. Kumar, A. Bharadwaj, M. Saini, G. Kardon, and S.J. Mathew. (2020) Myosin heavy chain-embryonic regulates skeletal muscle differentiation during mammalian development. Development 147(7). pii: dev184507. doi: 10.1242/dev.184507.
  5. Das S, P Kumar, A Verma, TK Maiti, and Mathew SJ (2019) Myosin heavy chain mutations that cause Freeman-Sheldon syndrome lead to muscle structural and functional defects in Drosophila Developmental Biology | 449(2): 90-98.
  6. M Saini , A Verma, SJ Mathew. (2018) SPRY2 is a novel MET interactor that regulates metastatic potential and differentiation in rhabdomyosarcoma. Cell Death and Disease 9:237
  7. Agarwal M, Kumar P, Mathew SJ (2015)  The Groucho/Transducin-like enhancer of split protein family in animal development. IUBMB Life 67:472.
  8. Keefe AC, Lawson JA, Flygare SD, Fox ZD, Colasanto MP, Mathew SJ, Yandell M, Kardon G. (2015) Muscle stem cells contribute to myofibres in sedentary adult mice. Nature Communications 14;6:7087 (Recommended by Faculty of 1000).
  9. Mathew SJ, Rembold M, Leptin M. (2011) A role for Traf4 in polarizing adherens junctions as a prerequisite for efficient cell shape changes. Molecular and Cellular Biology 24:4978.
  10. Murphy MM, Lawson JA, Mathew SJ, Hutcheson DA, Kardon G. (2011) Satellite cells, connective tissue fibroblasts and their interactions are crucial for muscle regeneration. Development 138:3625-3637. (Featured article, Recommended by Faculty of 1000)
  11. Mathew SJ. (2011) InACTIVatINg cancer cachexia. Dis Model Mech 4:283.
  12. Mathew SJ, Hansen JM, Merrell AJ, Murphy MM, Lawson JA, Hutcheson DA, Hansen MS, Angus-Hill M, Kardon G. (2011) Connective tissue fibroblasts and Tcf4 regulate myogenesis. Development 138:371-384.
  13. Mathew SJ, Haubert D, Kronke M, Leptin M. (2009) Looking beyond death: a morphogenetic role for the TNF signalling pathway. J Cell Sci 122:1939.
  14. Mathew SJ, Kerridge S, Leptin M. (2009) A small genomic region containing several loci required for gastrulation in Drosophila. PLoS One 4:e7437

Dr. Sam J Mathew
Associate Professor
Regional Centre for Biotechnology
NCR Biotech Science Cluster
3rd Milestone, Faridabad-Gurgaon Expressway
P.O. Box No. 3, Faridabad - 121 001
Haryana (NCR Delhi), India
E-mail: sjmathew at rcb dot res dot in
Phone: 91 129-2848822

Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 5 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 2 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 3 Grouped Photo 4 Grouped Photo 5 Grouped Photo 2 Grouped Photo 2 Grouped Photo 3