Disease Biology Laboratory

Major Focus:
• Investigating Signaling Pathways of Thrombosis, Inflammation and Adaptive Immune Responses in Human Diseases including Viral Infections to Polymorphism Associated Pathogenesis
• Identifying Biomarkers and Molecular Targets
• Developing Potential Therapeutics.

Viral Infections

Dengue virus (DENV), Japanese encephalitis virus (JEV) and Chikungunya virus (CHIKV) infection:
• Investigating diseases pathogenesis and role of platelet factor 4 (PF4 or CXCL4) using PF4-/-, PF4oe, CXCR3-/- mice, crossed with IFNα/β/γR-/- or IFNα/βR-/- mice.
• Investigating role of IgG/IgM and FcR interactions in primary and secondary infection of DENV using FcγRII-/- mice.
• Role of Glycosylation of IgG in severity of Antibody-dependent enhancement (ADE) in in secondary DENV infection in mice and human.
• Developing antivital small molecules against CXCL4-CXCR3 and testing in mice DENV models

Related pub: Gaur K et al. 2024 EMBO Molecular Medicine, Singh A et al. 2023 Journal of Thrombosis and Haemostasis; Singh A et al. 2023 Cytokine; Ojha et al. 2019 EBioMedicine.

SARS-CoV-2:

• Investigating pathogenesis of Acute Respiratory Distress Syndrome (ARDS) and Hypoxemia, and Pulmonary Fibrosis in SARS-CoV-2 infected animals and patients: Role of Akt, p38 and HIF1α, and epithelial to mesenchymal transition (EMT) signaling.
• Developing antivirals against Covid-19 and testing in mice models.

Related pub: Agarwal S et al. 2022. Clinical Translation Medicine; Shrimali NM et al. 2021 EBioMedicine

Severity of Viral infections in Diabetes

• Severity of SARS-CoV-2 infection and host immune response in mice with type 1 diabetes (T1D)/ type 2 diabetes (T2D).
• Severity of DENV infection and host immune response in mice with T1D/T2D and T2D patients.
• Examining the role of antiviral/antiglycemic role of Metformin in Covid-19/Dengue in diabetes.
• Investigating the role of CXCL4 in the severity of viral infection in diabetes.

Human papillomavirus (HPV)

• Understanding the correlates (including Glycosylation of IgG) of vaccine-induced antibody response: an Indian cohort study involving HPV vaccines.

Diabetes

Investigating pathogenesis of inflammation, thrombosis and pulmonary fibrosis

• Hyperglycemia induced thrombosis, thrombophilia and inflammation in mice and patients with T1D/T2D.
• Pulmonary fibrosis in mice with T1D/T2D: EMT signaling.
• Platelet and immune cells interactions contribute to thrombo-inflammatory complications in mice and patients with T2D.
• Hyperglycemia induced glycosylation of CD36 in platelets promotes oxLDL-mediated platelet hyperactivation and thrombosis in T2D patients: Lyn-JNK signaling.

Related pub: Agarwal S et al. 2023 FEBS Journal; Agarwal S et al. 2023 Clinical Experimental Immunology.

Genetic polymorphism

PHD2D4E;C127S polymorphisms in Tibetans and High-altitude Adaptation

• Tibetan highlanders carrying PHD2D4E;C127S polymorphisms are protected from polycythemia and Edema, on the other hand, non-Tibetan travellers from sea level to high altitudes are predisposed to mountain maladies like pulmonary and cerebral edema: Role of PHD2, HIF1α and Akt signaling.
• Tibetans carrying PHD2D4E;C127S polymorphisms are protected from hypoxia induced thrombosis and thrombophilia? Role of PHD2, HIF1α and Akt signaling.
•Tibetans carrying PHD2D4E;C127S polymorphisms are protected from viral infections at high altitude under hypoxia? Role of PHD2, HIF1α and Akt signaling.
• Metabolome profiles of glucose and fatty acid oxidation pathways in plasma/blood cells in T2D patients from high altitudes are different sea level counterparts? Does high-altitude hypoxia and PHD2, HIF1α signaling play role? Does gut microbiome play role? Do hypoxia-responsive factors crosstalk with metabolic enzymes to alter metabolome profiles of T2D patients at high altitude? Genetic polymorphism and/or epigenetic modifications of metabolic enzymes play role?

Related pub: Bhattacharya S et al. 2021 EBioMedicine; Sharimali NM et al. 2021 EBioMedicine.

FBLN4D203A polymorphisms

Investigating role of Lethal Aortopathy syndrome associated with novel FBLN4D203A Mutation among Mappila Children of Malabar, Kerala - seeking novel clinical, molecular genetic and anthropological insights among Mappila Children of Malabar, Kerala.

Kashmiri Thrombocytopenia

About 20% of Healthy Individuals from Kashmir Valley have lower platelet counts (<90). We are investigating if any genetic polymorphisms are associated with it.

Hemolytic disorders

•Platelet-immune cells crosstalk in the pathogenesis of systemic inflammation in hemolytic disorders including paroxysmal nocturnal hemoglobinuria (PNH) and sickle cell disease (SCD): Role of extracellular hemoglogin (Hb).

Related pub: Sighal R et al. 2015 Haematologica; Sighal R et al. 2016 Clinical Immunology; Sighal R et al. 2018 Euro J Immunology; Sighal R et al. 2019 Journal Immunology Research; Bhasym A et al. 2019 Clinical Experimental Immunology.

Autoimmune disorders

• Systemic inflammation in autoimmune disorders like atypical hemolytic uremic syndrome (aHUS) and systemic lupus erythematosus (SLE): Role of complement factors H polymorphisms. • Thrombophilia or hypercoagulation in aHUS and SLE: Crosstalk between complement and coagulation factors.

Related pub: Bhasym A et al. 2019 Journal of Clinical Immunology

• Kishan K Gaur
  Senior Research Fellow
  kishan.gaur@rcb.res.in
• Garima Joshi
  Senior Research Fellow
  garima.joshi@rcb.res.in
• Garima Verma
  Senior Research Fellow
  garima.verma@rcb.res.in
• Tejeswara Rao Asuru
  Junior Research Fellow
  tejeswara.rao@rcb.res.in
• Shalu Singh
  Junior Research Fellow
  shalu.singh@rcb.res.in
• Md. Sahil
  Junior Research Fellow
  md.sahil@rcb.res.in
• Anupam Chawla
  Junior Research Fellow
  anupam.chawla@rcb.res.in
• Sakshi Mandal
  Junior Research Fellow
  sakshi.mandal@rcb.res.in
• Riya Ghosh
  Junior Research Fellow-Project
  riya.ghosh@rcb.res.in
• Navya Chauhan
  Junior Research Fellow-Project
  navya.chauhan@rcb.res.in
• Gulistan Parveen
  Junior Research Fellow-Project
  gulistan.parveen@rcb.res.in
• Richa Kumari
  Junior Research Fellow-Project
  richa.kumari@rcb.res.in
• Aarti Muralitharan
  Junior Research Fellow-Project
  aarti.muralitharan@rcb.res.in
• Anupama
  MS-PhD Student
  anupama.msphd@rcb.res.in

Original Articles
1. Gaur KK, Asuru TR, Srivastava M, Singh N, Purushotham N, Poojary B, Das B, Bhattacharyya S, Asthana S, Guchhait P* (2024) 7D, a small molecule inhibits dengue infection by increasing interferons and neutralizing-antibodies via CXCL4:CXCR3:p38:IRF3 and Sirt1:STAT3 axes respectively. EMBO Molecular Medicine (in press).
2. Singh A, Ghosh R, Asuru TR, Prajapat S, Joshi G, Gaur KK, Shrimali NM, Ojha A, Vikram NK, Poncz M, Kalia M, Guchhait P* (2023) Inhibition of platelet factor 4:CXCR3 pathway prevents JEV and DENV infection. Journal of Thrombosis and Haemostasis, doi.org/10.1016/j.jtha.2023.11.015.
3. Agarwal S, Saha S, Ghosh R, Sarmadhikari S, Asthana S, Maiti TK, Khadgawat R, Guchhait P* (2023) Elevated glycosylation of CD36 in platelets is a risk factor for oxLDL mediated platelet activation in type 2 diabetes. FEBS Journal, https://doi.org/10.1111/febs.16976
4. Singh A, Ghosh R, Guchhait P* (2023) CXCR3 antagonist rescues ER stress and reduces inflammation and JEV infection in mice brain. Cytokine, 7:172:156380. 10.1016/j.cyto.2023.156380
5. Agarwal S, Ghosh R, Verma G, Khadgawat R, Guchhait P* (2023) Alpha-ketoglutarate supplementation reduces inflammation and thrombosis in type 2 diabetes by suppressing leukocytes and platelet activation. Clinical and Experimental Immunology, https://doi.org/10.1093/cei/uxad086
6. Agarwal S, Kaur S, Asuru TR, Joshi G, Shrimali NM, Singh A, Singh ON, Srivastva P, Shrivastava T, Vrati S, Surjit M, Guchhait P* (2022) Dietary αKG inhibits SARS CoV-2 infection and rescues inflamed lungs to restore normal O2 saturation by inhibiting pAkt. Clinical and Translational Medicine e1041,12(9):1-6.
7. Shrimali NM, Agarwal S, Kaur S, Bhattacharya S, Bhattacharyya S, Prchal JT, Guchhait P* (2021) KG inhibits thrombosis and inflammation by prolyl hydroxylase-2 mediated inactivation of phospho-Akt. EBioMedicine 73:103672.
8. Bhattacharya S, Shrimali NM, Mohammad G, Koul PA, Prchal JT and Guchhait P*. (2021) Gain-of-function Tibetan PHD2D4E;C127S variant suppresses monocyte function: a lesson in inflammatory response to inspired hypoxia. EBioMedicine 68:103418.
9. Bhasym A, Annarapu GK, Saha S, Shrimali NM, Gupta S, Seth T, Guchhait P*. (2019) Neutrophils develop rapid proinflammatory response after engulfing Hb-activated platelets under intravascular hemolysis. Clinical and Experimental Immunology, doi: 10.1111/cei.13310
10. Bhasym A, Gurjar BS, Sriharsha TM, Prabhu S, Puraswani M, Khandelwal P, Saini H, Saini S, Verma AK, Chatterjee P, Bal V, George A, Sharma A, Hari P, Sinha A, Bagga A, Rath S, Guchhait P* (2019) Altered Peripheral Blood Leucocyte Phenotype and Responses in Healthy Individuals with Homozygous Deletion of FHR1 and FHR3 Gene. Journal of Clinical Immunology, doi.org/10.1007/s10875-019-00619-2
11. R Singhal, DK Rathore, T Bhakuni, T Seth, P Guchhait* (2019) Absence of Non-classical Monocytes in Hemolytic Patients: Free Hb and NO-Mediated Mechanism. Journal of Immunology Research, doi.org/10.1155/2019/1409383
12. Ojha A, Bhasym A, Mukherjee S, Annarapu GK, Bhakuni T, Akbar I, Seth T, Vikram NK, Vrati S, Basu A, Bhattacharyya S, Guchhait P* (2019) Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses. EBioMedicine, 39: 332-347
13. Singhal R, Chawla S, Batra H, Gupta S, Ojha A, Rathore DK, Seth T and Guchhait P* (2018) Engulfment of Hb-activated platelets differentiates monocytes into pro-inflammatory macrophages in PNH patients. European Journal of Immunology. 48(8):1285-1294 doi: 10.1002/eji.201747449.
14. Singhal R, Chawla S, Rathore D, Bhasym A, Annarapu GK, Sharma V, Seth T, Guchhait P* (2016) Development of pro-inflammatory phenotype in monocytes after engulfing Hb-activated platelets in hemolytic disorders. Clinical Immunology, doi:10.1016/ j.clim.2016.12.007.
15. Singhal R, Annarapu GK, Pandey A, Chawla S, Ojha A, Gupta A, Cruz MA, Seth T, Guchhait P* (2015) Hemoglobin interaction with GP1b-alpha induces platelet activation and apoptosis: a novel mechanism associated with intravascular hemolysis. Haematologica 100(12): 1526-33.
16. Da Q, Teruya M, Guchhait P, Teruya J, Olson JS, Cruz MA*. (2015) Free hemoglobin increases von Willebrand factor-mediated platelet adhesion in vitro: implications on circulatory devices. Blood. 126(20):2338-41.
17. Lorenzo LR, Huff C, Myllymäki M, Olenchock B, Swierczek S, Tashi T, Gordeuk V, Wuran T, Li GR, McClain DL, Khan TM, Koul PA, Guchhait P, Salama ME, Xing J, Semenza GL, Liberzon E, Wilson A, Simonson TS, Jorde LB, Kaelin Jr WG, Koivunen P & Prchal JT* (2014) A genetic mechanism for Tibetan high-altitude adaptation. Nature Genetics, 46:951-956
18. Zhou Z, Thiagarajan P, Udden MA, Lopez JA, Guchhait P* (2011) Membrane sulfatide plays a crucial role in the sickle erythrocytes adhesion to matrix and endothelial ligands. Thrombosis and Haemostasis. 105:1046-52.
19. Zhou Z, Han H, Cruz MA, Jose JA, Dong JF, Guchhait P* (2009) Hemoglobin blocks von Willebrand factor proteolysis by ADAMTS-13: a mechanism associated with sickle cell disease. Thrombosis and Haemostasis, 101 (6): 1070-77.
20. Guchhait P*, Shrimpton C, Honke K, Rumbaut R, Lopez JA, Thiagarajan P (2008) Effects of an anti-sulfatide single-chain antibody probe on platelet function. Thrombosis and Haemostasis, 99(3):552-57.

Original Articles
1. Gaur KK, Asuru TR, Srivastava M, Singh N, Purushotham N, Poojary B, Das B, Bhattacharyya S, Asthana S, Guchhait P* (2024) 7D, a small molecule inhibits dengue infection by increasing interferons and neutralizing-antibodies via CXCL4:CXCR3:p38:IRF3 and Sirt1:STAT3 axes respectively. EMBO Molecular Medicine (in press).
2. Singh A, Ghosh R, Asuru TR, Prajapat S, Joshi G, Gaur KK, Shrimali NM, Ojha A, Vikram NK, Poncz M, Kalia M, Guchhait P* (2023) Inhibition of platelet factor 4:CXCR3 pathway prevents JEV and DENV infection. Journal of Thrombosis and Haemostasis, 22:3:818-833 doi.org/10.1016/j.jtha.2023.11.015
3. Agarwal S, Saha S, Ghosh R, Sarmadhikari S, Asthana S, Maiti TK, Khadgawat R, Guchhait P* (2023) Elevated glycosylation of CD36 in platelets is a risk factor for oxLDL mediated platelet activation in type 2 diabetes. FEBS Journal,
4. Singh A, Ghosh R, Guchhait P* (2023) CXCR3 antagonist rescues ER stress and reduces inflammation and JEV infection in mice brain. Cytokine, 7:172:156380. 10.1016/j.cyto.2023.156380
5. Agarwal S, Ghosh R, Verma G, Khadgawat R, Guchhait P* (2023) Alpha-ketoglutarate supplementation reduces inflammation and thrombosis in type 2 diabetes by suppressing leukocytes and platelet activation. Clinical and Experimental Immunology,
6. Kumari Sharda, Bandyopadhyay Bhaswati, Singh Anamika, Aggarwal Suruchi, Yadav Amit, Vikram Naval, Guchhait Prasenjit and Banerjee Arup (2023) Extracellular vesicles recovered from plasma of severe dengue patients induced CD4+T cell suppression through PD-L1/PD1 interaction. mBIO (accepted).
7. Gupta A, Sood A, Bhardwaj D, Shrimali N, Singhmar R, Chaturvedi S, Guchhait P, Agrawal G* (2023) Functionalized Chitosan Decorated Hafnium Oxide@Gold Core-Shell Nanoparticles for Multimodal Cancer Therapy. Advanced Therapeutics (accepted).
8. Agarwal S, Kaur S, Asuru TR, Joshi G, Shrimali NM, Singh A, Singh ON, Srivastva P, Shrivastava T, Vrati S, Surjit M, Guchhait P* (2022) Dietary αKG inhibits SARS CoV-2 infection and rescues inflamed lungs to restore normal O2 saturation by inhibiting pAkt. Clinical and Translational Medicine e1041,12(9):1-6.
9. Gupta A, Sood A, Dhiman A, Shrimali N, Singhmar R, Guchhait P, Agarwal G*. (2022) Redox Responsive Poly(allylamine)/Eudragit S-100 Nanoparticles for Dual Drug Delivery in Colorectal Cancer. Biomaterials Advances 143:e213184.
10. Thakur SK, Ghosh R, Gaur KK, Guchhait P, Eswaran SV* (2022) Anti-cancer activity of water-soluble reduced Graphene oxide-aryl arizidino manganese II complex. J Biomedical Nanotechnology 18:1-8.
11. Mohanbhai SJ, Sardoiwala MN, Gupta S, Shrimali NM, Roy Choudhury S, Sharma SS, Guchhait P, Karmakar S* (2022) Colon targeted chitosan-melatonin nanotherapy for preclinical inflammatory bowel disease. Biomaterials advances 136:e212796.
12. Gautam D, Kailashiya J, Tiwari A, Chaurasia RN, Annarapu GK, Guchhait P, Dash D* (2022) Fibrinogen mitigates prion-mediated platelet activation and neuronal cell toxicity. Frontiers Cell and developmental Biology, 10:e834016.
13. Kaur S, Singh A, Kaur J, Verma N, Pandey AK, Das S, Bhattacharyya S, Guchhait P* (2022) Upregulation of cytokine signaling in platelet increases risk of thrombophilia in severe COVID-19 patients. Blood Cells Molecules and Diseases, 94:102653.
14. Chaudhury N, Srivastava S, Dave U, Ojha A, Guchhait P, Chandele A, Patel AK*. (2022). High-mobility group box 1 protein promotes dengue virus replication by interacting with untranslated regions of viral genome. Virus Research, 309:198668.
15. Shrimali NM, Agarwal S, Kaur S, Bhattacharya S, Bhattacharyya S, Prchal JT, Guchhait P* (2021) KG inhibits thrombosis and inflammation by prolyl hydroxylase-2 mediated inactivation of phospho-Akt. EBioMedicine 73:103672.
16. Bhattacharya S, Shrimali NM, Mohammad G, Koul PA, Prchal JT and Guchhait P*. (2021) Gain-of-function Tibetan PHD2D4E;C127S variant suppresses monocyte function: a lesson in inflammatory response to inspired hypoxia. EBioMedicine 68:103418.
17. Srivastava S, Chaudhary N, Ojha A, Guchhait P, Patel AK*. (2021) Signal transducer and activator of transcription 3 (STAT3) acts as a proviral factor for dengue virus propagation. Virus Research 300:198436
18. Thakur S, Saha S, Guchhait P, Eswaran S*. (2020) Fullarazirman, nano water-soluble Fullerene C60-Mn (II) Triadduct complex with anti-cancer activity comparable to Doxorubicin. J Biomedical Nanotechnology. 16:481-491.
19. Thakur S, Goswami K, Rao P, Kaushik S, Singh B, Sharma PK, Asthana S, Bhattacharjee S, Guchhait P, Eswaran S*. (2020) A Fluoresceinated Aminohexanol Tethered Inositol Hexakisphosphate: Studies on Arabidopsis thaliana, Drosophila melanogaster and Docking with 2P1M Receptor. ACS Omega, 0c00961
20. Sardoiwala N M, Kushwaha A, Dev A, Nishith S, Guchhait P, Karmakar S, Roy Choudhury S*. (2020) Hypericin loaded transferrin nanoparticles induce PP2A regulated BMI1 degradation in colorectal cancer specific chemo-Photodynamic Therapy. ACS Biomaterials Science & Engineering, 6,5, 3139-3153.
21. Sood A, Dev A, Mohanbhai SJ, Shrimali N, Kapasiya M, Kushwaha A, Choudhury SR, Guchhait P, Karmakar S* (2019) Development and targeting efficacy of disulfide bridged chitosan-Eudragit S-100 nanoparticles for colorectal cancer, ACS Applied Nano Materials. 2, 10: 6409-17.
22. Thakur S, Goswami K, Bhattacharjee S, Soni U, Guchhait P, Eswaran S*. (2019) A Water Soluble Single Walled Carbon Nanotube Aryl Aziridino Carboxylic Acid Decorated Mn (II) Complex Increased Root Growth in Arabidopsis thaliana. Chemistry Select. 201904205.
23. Bhasym A, Bhakuni T and Guchhait P* (2019) Elevated surface-bound complement factor-H alters the function of platelets and monocytes in FHR1/3-null healthy individuals. Blood Cells Molecules Diseases, 79:102349.
24. Bhasym A, Annarapu GK, Saha S, Shrimali NM, Gupta S, Seth T, Guchhait P*. (2019) Neutrophils develop rapid proinflammatory response after engulfing Hb-activated platelets under intravascular hemolysis. Clinical and Experimental Immunology, doi: 10.1111/cei.13310
25. Bhakuni T, Singhal R, Aannarapu GK, Sharma A, Mahapatra M, Saxena R, Guchhait P* (2019) Unique case of autoantibody mediated inactivation of ADAMTS13 in an Indian TTP patient. Blood Cells Molecules Diseases, 77:29-33
26. Bhasym A, Gurjar BS, Sriharsha TM, Prabhu S, Puraswani M, Khandelwal P, Saini H, Saini S, Verma AK, Chatterjee P, Bal V, George A, Sharma A, Hari P, Sinha A, Bagga A, Rath S, Guchhait P* (2019) Altered Peripheral Blood Leucocyte Phenotype and Responses in Healthy Individuals with Homozygous Deletion of FHR1 and FHR3 Gene. Journal of Clinical Immunology, doi.org/10.1007/s10875-019-00619-2
27. R Singhal, DK Rathore, T Bhakuni, T Seth, P Guchhait* (2019) Absence of Non-classical Monocytes in Hemolytic Patients: Free Hb and NO-Mediated Mechanism. Journal of Immunology Research, doi.org/10.1155/2019/1409383
28. Ojha A, Bhasym A, Mukherjee S, Annarapu GK, Bhakuni T, Akbar I, Seth T, Vikram NK, Vrati S, Basu A, Bhattacharyya S, Guchhait P* (2019) Platelet factor 4 promotes rapid replication and propagation of Dengue and Japanese encephalitis viruses. EBioMedicine, 39: 332-347
29. Singhal R, Chawla S, Batra H, Gupta S, Ojha A, Rathore DK, Seth T and Guchhait P*. (2018) Engulfment of Hb-activated platelets differentiates monocytes into pro-inflammatory macrophages in PNH patients. European J Immunology. 48(8):1285-1294 doi: 10.1002/eji.201747449.
30. Gurjar BS, Sriharsha TM, Bhasym A, Prabhu S, Puraswani M, Khandelwal P, Saini H, Saini S, Verma AK, Chatterjee P, Guchhait P, Bal V, George A, Rath S, Sahu A, Sharma A, Hari P, Sinha A, Bagga A. (2018) Characterization of genetic predisposition and autoantibody profile in atypical hemolytic uremic syndrome. Immunology. 154, 663-672. doi: 10.1111/imm.12916.
31. Ojha A, Nandi D, Batra H, Singhal R, Annarapu GK, Bhattacharyya S, Seth T, Dar L, Medigeshi GR, Vrati S, Vikram NK, Guchhait P*. (2017) Platelet activation determines the severity of thrombocytopenia in dengue infection. Scientific Reports, 7:41697.
32. Tashi T, Reading NS, Moore LG, Wuren T, Hu H, Teng F, Shestakova A, Lorenzo F, Burjanivova T, Koul P, Guchhait P, Wittwer C, Julian CG, Shah B, Huff CD, Ge R, Gordeuk VR, Prchal JT*. (2017) Gain-of-Function EGLN1 Prolyl Hydroxylase (PHD2 D4E:C127S) in Combination with EPAS1 (HIF2α) Polymorphism Lowers Hemoglobin Concentration in Tibetans. Journal of Molecular Medicine, p1-6.
33. Singhal R, Chawla S, Rathore D, Bhasym A, Annarapu GK, Sharma V, Seth T, Guchhait P*. (2016) Development of pro-inflammatory phenotype in monocytes after engulfing Hb-activated platelets in hemolytic disorders. Clinical Immunology, doi:10.1016/ j.clim.2016.12.007.
34. Annarapu GK, Singhal R, Gupta A, Chawla S, Batra H, Seth T, and Guchhait P*. (2016) HbS binding to GP1bα activates platelets in sickle cell disease. PLoS One, 11(12): e0167899. doi:10.1371/journal.
35. Annarapu GK, Singhal R, Peng Y, Guchhait P*. (2016) Inhibition of Hb binding to GP1bα abrogates Hb-mediated thrombus formation on immobilized VWF and collagen under physiological shear stress. PLoS One, 11(4):e0154276.
36. Singhal R, Annarapu GK, Pandey A, Chawla S, Ojha A, Gupta A, Cruz MA, Seth T, Guchhait P*. (2015) Hemoglobin interaction with GP1balpha induces platelet activation and apoptosis: a novel mechanism associated with intravascular hemolysis. Haematologica 100(12): 1526-33.
37. Da Q, Teruya M, Guchhait P, Teruya J, Olson JS, Cruz MA*. (2015) Free hemoglobin increases von Willebrand factor-mediated platelet adhesion in vitro: implications on circulatory devices. Blood. 126(20):2338-41.
38. Lorenzo LR, Huff C, Myllymäki M, Olenchock B, Swierczek S, Tashi T, Gordeuk V, Wuran T, Li GR, McClain DL, Khan TM, Koul PA, Guchhait P, Salama ME, Xing J, Semenza GL, Liberzon E, Wilson A, Simonson TS, Jorde LB, Kaelin Jr WG, Koivunen P & Prchal JT*. (2014) A genetic mechanism for Tibetan high-altitude adaptation. Nature Genetics, 46:951-956.
39. Zhou Z, Thiagarajan P, Udden MA, Lopez JA, Guchhait P*. (2011) Membrane sulfatide plays a crucial role in the sickle erythrocytes adhesion to matrix and endothelial ligands. Thrombosis Haemostasis. 105:1046-52.
40. Zhou Z, Han H, Cruz MA, Jose JA, Dong JF, Guchhait P*. (2009) Hemoglobin blocks von Willebrand factor proteolysis by ADAMTS-13: a mechanism associated with sickle cell disease. Thrombosis Haemostasis, 101 (6): 1070-77.
41. Guchhait P*, Shrimpton C, Honke K, Rumbaut R, Lopez JA, Thiagarajan P. (2008) Effects of an anti-sulfatide single-chain antibody probe on platelet function. Thrombosis Haemostasis, 99(3):552-57.
42. Dasgupta SK, Abdel-Monem H, Guchhait P, Nagata S, Thiagarajan P*. (2008) Role of lactadherin in the clearance of phosphatidylserine-expressing red blood cell in sickle cell disease. Transfusion, 48(11):2370-76.
43. Guchhait P, Dasgupta SK, Le A, Yellapragada S, Lopez JA, Thiagarajan P*. (2007) Lactadherin mediates sickle cell adhesion to the vascular endothelial cells in flowing blood. Hematologica, 92(9):1266-67.
44. Dasgupta SK, Guchhait P, Thiagarajan P*. (2006) Lactadherin binding and phosphatidylserine expression on cell surface-comparison with annexin A5. Translational Research, 148:19-25.
45. Lasser G, Guchhait P, Ellsworth J, Bishop P, Cruz MA, Lopez JA, Fruebis J*. (2006) C1q-TNF related protein-1 (CTRP-1), a vascular wall protein that inhibits collagen-induced platelet aggregation by blocking VWF binding to collagen. Blood, 107:423-430.
46. Guchhait P, Lopez JA, Thiagarajan P*. (2004) Characterization of autoantibodies against sulfatide from a V-gene phage-display library derived from patients with systemic lupus erythematosus. J. Immunol. Methods, 295: 129–137.
47. Bernardo A, Bergeron AL, Sun CW, Guchhait P, Cruz MA, Lopez JA, Dong JF*. (2004) Von Willebrand factor present in fibrillar collagen enhances platelet adhesion to collagen and collagen-induced platelet aggregation. J. Thromb. Haemost. 2(4): 660-9.
48. Guchhait P, Tosi MF, Smith CW, Chakraborty A*. (2003) The murine myeloid cell line 32Dcl3 as a model system for studying neutrophil functions. J. Immunol. Methods. 283(1-2): 195-204.

Review Articles

1. Chauhan N, Gaur KK, Asuru TR, Guchhait P* (2024) Dengue virus: pathogenesis and potential small molecule antivirals. Bioscience Reports, 44 BSR20240134; https://doi.org/10.1042/BSR20240134
2. Joshi G, Das A, Verma G, Guchhait P* (2023) Viral infection and host immune response in diabetes. IUBMB Life, DOI: 10.1002/iub.2794.
3. Shrimali MN, Agarwal S, Tiwari A, Guchhait P* (2022) Platelet-Neutrophil interactions and thrombo-inflammatory complications in type-2 diabetes mellitus. Current Physiology Reports, 10:1-10
4. Bhattacharya S, Agarwal S, Shrimali SM and Guchhait P* (2021) Interplay between hypoxia and inflammation contributes to the progression and severity of respiratory viral diseases. Molecular Aspects of Medicine. 81:101000
5. Singh A, Bisht P, Bhattacharya S, Guchhait P*. (2020) Role of platelet cytokines in Dengue virus infection. Frontiers in Cellular and Infection Microbiology, 10:561366
6. Batra H, Chawla S, Singhal R, Annarapu GK, Guchhait P*. (2016) Cell free hemoglobin modulates phenotype and function of immune cells in hemolytic disorders. Austin Hematology, 1(1): 1007.
7. Bhattacharya S, Bhasym A, Guchhait P*. (2016) Insights into Cardiovascular Diseases: The Vicious Platelet-Immune System Loop. Cardiology: Open Access, 1(1):1-7.
8. Annarapu GK, Singhal R, Chawla S, Ojha A, Guchhait P*. (2016) Hemoglobin mediated regulation of platelet functions. Journal of Hematology and Transfusion, 4(3): 1052.
9. Pandey A, Chawla S, Guchhait P*. (2015) Type-2 diabetes: Current understanding and future perspectives. IUBMB Life. 67:506.
10. Chawla S., Singhal R., Guchhait P*. (2014) Hypertension in hypoxia. Hypertension Cardiol. 1 (3): 1-16.
11. Zhou Z, Yee D, Guchhait P*. (2012) Molecular link between intravascular hemolysis and vascular occlusion in sickle cell disease. Current Vascular Pharmacology. 10(6):756-61.
12. Zhou Z, Behymer M, Guchhait P*. (2011) Role of extracellular hemoglobin in thrombosis and vascular occlusion in patients with sickle cell anemia. Anemia. 10.1155/2011/918916.
13. Zhou Zhou, Prasenjit Guchhait*. (2010) Extracellular Hemoglobin regulation of von Willebrand factor activity. US Oncology & Hematology, 7(2):150–2.
14. Zhou Z*, Nguyen T, Guchhait P, Dong JF*. (2010) Von Willebrand factor, ADAMTS-13, and Thrombotic thronbocytopenic purpura. Seminar in Thrombosis Hemostasis, 36:71-81.

Book Chapter

1. Tiwari RL, Annarapu GK, Guchhait P*. (2013) Pathophysiological consequences of hemolysis in sickle cell disease in "Sickle Cell Disease" (Ed. D Acuna-Costroviejo and I Rusanova). Nova Science Publishers, Inc. ISBN: 978-1-62618-774-0